DNA
comEF

Part:BBa_K233303:Design

Designed by: Praveen Kishore Sahu, Amrita Oak   Group: iGEM09_IBB_Pune   (2009-09-20)


Competence factors E & F


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 965
    Illegal XbaI site found at 195
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 966
    Illegal PstI site found at 980
    Illegal NotI site found at 7
    Illegal NotI site found at 973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 966
    Illegal XbaI site found at 195
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal XbaI site found at 195
    Illegal SpeI site found at 966
    Illegal PstI site found at 980
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Normal. ComE and Com F conjugated sequence.


Source

Acinetobacter BD413 sp

References